[Search for actinomycetes producing phospholipase C inhibitors]

The inhibiting activity of the cultural broth filtrate against phospholipase C was studied in 2920 actinomycetes. The inhibitor which did not bind to blood proteins was produced by 12 cultures. Streptomyces saraceticus, strain 0845, had the highest antiphospholipase activity. The activity was detected in the cultural broth of S. saraceticus by the first day of its growth and reached the maximum by the third day. Soybean flour and sucrose were shown to be the best sources of nitrogen and carbon for the manifestation of the inhibiting activity against phospholipase C. The culture responded to the level of aeration in the medium and the most intensive inhibitor synthesis was found at the oxygen solution rate of 0.84 to 1.32 g O2 per litre per hour. The inhibiting activity was preserved when the cultural broth filtrate was kept at room temperature and pH from 4.0 to 11.5 for 3 hours.