Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RBBP6 and cancer: from molecular mechanisms to clinical implications.

Frontiers in oncology·2026
Same author

Cancer-derived exosomes: mediators of immune crosstalk and emerging targets for immunotherapy.

Frontiers in immunology·2025
Same author

Advances in CRISPR-Cas systems for blood cancer.

Progress in molecular biology and translational science·2024
Same author

Development of a Plant-Expressed Subunit Vaccine against Brucellosis.

Microorganisms·2024
Same author

Novel strategies for drug repurposing.

Progress in molecular biology and translational science·2024
Same author

Protocol for Testing the Effects of ssDNA Aptamer in HeLa and MCF-7.

Methods in molecular biology (Clifton, N.J.)·2024

Related Experiment Video

Updated: May 21, 2025

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

27.2K

Methodology for Assessing Drug Efficacy: Protocol for Single and Combination Drug Screening Using HeLa Cell Cultures.

Phumuzile Dube1,2, Bernice Monchusi1,3, Mutsa M Takundwa1,2

  • 1Synthetic Nanobiotechnology and Biomachines, Synthetic Biology and Precision Medicine Centre, Future Production Chemicals Cluster, Council for Scientific and Industrial Research, Pretoria, South Africa.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2025
PubMed
Summary

This study details a drug sensitivity testing (DST) protocol for HeLa cells, evaluating single and combination drug effectiveness for cancer research and precision medicine. The method optimizes drug combinations to improve therapeutic outcomes and reduce toxicity.

Keywords:
Combination drug assayDrug Sensitivity Testing (DST)HeLa cellsPersonalized medicine

More Related Videos

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

2.5K
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
00:12

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

8.4K

Related Experiment Videos

Last Updated: May 21, 2025

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

27.2K
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

2.5K
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
00:12

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

8.4K

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Drug sensitivity testing (DST) is crucial for cancer research and functional precision medicine.
  • Understanding individual drug responses and optimizing drug combinations are key to effective cancer therapy.
  • Existing methods may require refinement for scalability and precise analysis of drug interactions.

Purpose of the Study:

  • To establish a detailed protocol for drug sensitivity testing (DST) on HeLa cells.
  • To evaluate both single-drug and combination-drug screening for assessing cell viability.
  • To provide a framework for identifying optimal drug combinations with minimized toxicity and enhanced efficacy.

Main Methods:

  • Preparation and maintenance of HeLa cell cultures.
  • Seeding cells in 96-well plates for low-throughput screening.
  • Performing single and combination drug treatments, followed by cell viability assessment.
  • Utilizing open-source tools like BREEZE and Synergy-Finder for data analysis.

Main Results:

  • The protocol enables precise analysis of cell viability in response to various drug treatments.
  • It facilitates the evaluation of synergistic interactions between drug combinations.
  • The method demonstrates scalability and reproducibility for DST applications.

Conclusions:

  • This robust DST protocol offers a reproducible framework for cancer research.
  • It is applicable to diverse cell lines, patient samples, and drug types.
  • The protocol supports the development of improved clinical treatment strategies through precise drug response analysis.