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Updated: May 21, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Photoperiod-mediated rapid generation advancement in soybean (Glycine max (L.) Merr.)
Ambika Rajendran1, Ayyagari Ramlal2,3, Dhandapani Raju4,5
1Division of Genetics, ICAR-Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, 110012, India. rambikarajendran@gmail.com.
Abstract:
Soybean is a short-day crop and the long-duration variety takes 120 days for maturity. A protocol for rapid generation advancement in soybean breeding is worthwhile keeping in view its utility. The study emphasizes standardisation of physical conditions, especially using warm white and cool white light-emitting diodes to hasten flowering and pod setting in soybean (DS9712 genotype). Complete open flowers were obtained with a 16 L/8D (dark/light) photoperiod in 30 days. The results also highlighted the application of interventions of physical conditions and inputs, especially during the reproductive phase to shorten the seed-to-seed generation time by around 15 days in a low-cost method for soybean breeding. Breeding will be revolutionised in case economic speed breeding is combined with modern breeding technologies, thereby resulting in more generations per year.
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