Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Combination of Mechanical Treatment and Enzymatic Hydrolysis During Post-Consumer Cotton Waste Processing.

ChemSusChem·2026

Same author

A Method for Measuring Speech Intelligibility Using Connected Speech.

Journal of speech, language, and hearing research : JSLHR·2026

Same author

Acoustic Scene-Aware Processing and Auditory Model-Based Compensation Strategies.

Journal of the Association for Research in Otolaryngology : JARO·2026

Same author

Patient-Derived Immortalized Limbal Epithelial Cells as In Vitro Models of Congenital Aniridia.

Cells·2026

Same author

Investigating the impact of background noise on collaborative decision-making using an individual-weighted voting model.

Cognitive research: principles and implications·2026

Same author

Establishment and characterization of a human juvenile bone marrow-derived mesenchymal stem/stromal cell line under advanced culture conditions for osteogenic differentiation.

Frontiers in bioengineering and biotechnology·2026

Search research articles

Related Experiment Video

Updated: May 2, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Automated sample tracking and parameter adaption for scanning laser optical tomography.

Hannes Benecke¹, Firas Almadani¹, Johannes Heske¹

¹Life Sciences Department, Laser Zentrum Hannover e.V, Hannover, Germany.

|March 19, 2025

Summary

This summary is machine-generated.

Automated software corrects sample alignment in Scanning Laser Optical Tomography. This digital tracking and motion artifact correction improves imaging quality, reliability, and system accessibility for 3D imaging applications.

More Related Videos

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Related Experiment Videos

Last Updated: May 2, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Area of Science:

Medical imaging
Materials science
Optical engineering

Background:

Non-destructive 3D imaging is crucial for medical and technical analysis.
Scanning Laser Optical Tomography (SLOT) requires manual sample alignment for high-quality results.
Manual alignment in SLOT is user-dependent, leading to inconsistent image quality.

Purpose of the Study:

To develop a software-based automation technique for sample alignment in SLOT.
To improve the quality, reliability, and automation of SLOT imaging.
To make advanced 3D imaging techniques more accessible.

Main Methods:

Digital detection and tracking of the sample during acquisition, replacing physical alignment.
Implementation of a secondary automation algorithm to correct residual motion artifacts.
Integration of these algorithms to enhance tomographic reconstruction.

Main Results:

Significant improvement in the quality of 3D imaging.
Increased reliability and repeatability of imaging results.
Enhanced degree of automation for the SLOT system.

Conclusions:

Software-based automation effectively addresses manual alignment challenges in SLOT.
The developed technique improves imaging performance and broadens user accessibility.
This advancement contributes to more consistent and high-quality 3D imaging in various fields.