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Updated: May 21, 2025
Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Enhancement of dark fermentative hydrogen production using metal-modified biochar from sugarcane residues:
Nantharat Wongfaed1, Sureewan Sittijunda2, Sompong O-Thong3
1Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen, 40002, Thailand.
Abstract:
This study investigated the enhancement of dark fermentative hydrogen production (HP) using metal-modified biochars derived from sugarcane bagasse (SB) and sugarcane leaves (SL). The biochars were modified with Fe and Ni, with optimal conditions identified through Box-Behnken Design as 6.09 g/L biochar: SB, 5.38 g/L biochar: SL + Fe, and 7.66 g/L biochar: SL + Ni. This optimization achieved a maximum hydrogen yield of 108.77 mL-hydrogen (H2)/g-glucose, a 58.77 % increase over the control. Metal modification enhanced biochar surface properties and selectively enriched H2-producing bacteria, particularly Clostridium sensu stricto 1 and Paraclostridium sp. Metabolic pathway analysis showed enhanced glucose catabolism and increased H2-producing enzyme abundance. The study demonstrates that sugarcane-derived biochar can effectively enhance bio-HP, though careful optimization of metal concentrations is crucial to avoid inhibitory effects.
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