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Updated: May 21, 2025

Author Spotlight: Enhancing Microinjection Needle Quality by Wet Beveling
Published on: September 27, 2024
Needle sharpness is minimally affected by vaccine vial puncture
Julia R Tawil1, Emma C Vitello1, Gina M Agostini-Walesch2
11College of Veterinary Medicine, Midwestern University, Glendale, AZ.
Objective:
To survey the commonality of needle-changing practices among veterinarians and measure whether 1 or 2 passages through a vaccine vial septum affects needle sharpness.
Methods:
An anonymous online survey of needle-changing behaviors by veterinary professionals was conducted from April to May 2023. Subsequently, a blinded randomized controlled bench trial assessed the impact of septum passage on needle sharpness between June and August 2023 at Midwestern University. Veterinary hypodermic needles of 3 brands and 4 gauges were tested after passing through a septum 0, 1, or 2 times. The primary outcome was the peak penetration force (PF) required to puncture standardized material. Secondary analysis involved visual examination of needles with the use of an optical 3-D surface roughness measurement instrument.
Results:
Of 482 survey respondents, 76% routinely changed needles due to concerns about dulling. Experimental results showed a minor increase in PF after 1 (9%) and 2 (18%) septum passages. Needle gauge and brand impacted PF more than septum passage, with differences of 6% to 58% and 9% to 10%, respectively. Visual inspection revealed a 40% incidence of gross defects after 1 passage and 52% after 2 passages, with PF through the vial septum being the strongest damage predictor.
Conclusions:
While septum passage slightly increased the PF, brand and gauge had a more substantial impact.
Clinical Relevance:
This study suggests that changing needles may not be necessary for maintaining sharpness if proper technique is used, potentially reducing the risk of needlestick injuries and medical waste.

