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Related Experiment Video

Updated: May 21, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Electrochemically active DNA ligands for gene detection: present and future.

Shigeori Takenaka¹, Shinobu Sato²

¹Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, 804 8550, Japan. shige@che.kyutech.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|March 20, 2025

Summary

Electrochemical gene sensing uses DNA ligands for accurate diagnostics. Ferrocenyl naphthalene diimides (FNDs) enable sensitive detection of cancer markers and viral RNA, offering a reliable alternative to PCR.

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Published on: March 21, 2018

Area of Science:

Electrochemistry
Molecular Diagnostics
Biosensors

Background:

Electrochemical gene sensing is emerging as a powerful tool for diagnostic chips.
DNA ligands, including metal complexes, methylene blue, and ferrocenyl naphthalene diimide (FND), are crucial for these sensing methods.

Purpose of the Study:

To review electrochemical gene sensing methods based on electrochemically active DNA ligands.
To highlight the application of these methods in disease diagnosis and genetic analysis.

Main Methods:

Immobilization of DNA probes onto electrode surfaces.
Hybridization reactions with target DNA fragments.
Electrochemical detection using DNA ligands like FND, methylene blue, and FNC.

Main Results:

Successful cancer diagnosis via FND detection of hTERT gene methylation.
Electrochemical detection of PCR products using methylene blue (signal-off) and FNC (signal-on).
Electrochemical detection of telomerase activity and novel coronaviruses using FND and G-quadruplex (G4) interactions.

Conclusions:

Electrochemical gene sensing with DNA ligands offers a reliable and sensitive platform for diagnostics.
FNDs demonstrate versatility in detecting cancer markers and viral RNA through G4 structures.
This technology holds promise for developing rapid, non-invasive diagnostic tests, including point-of-care applications.