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Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

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Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
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Efficient clonal seeds sorting for apomictic hybrid rice using a pollen-specific gene switch system.

Yijie Zhan1, Yumei Xia2,3, Yao Wang4

  • 1Long Ping Branch, College of Biology, Hunan University, Changsha, China.

Plant Biotechnology Journal
|March 20, 2025
PubMed
Summary

A new pollen-specific gene switch (PSGS) system effectively distinguishes clonal from zygotic rice seeds. This advancement is crucial for improving apomixis in rice cultivation and ensuring clonal seed purity.

Keywords:
Cre/Loxp + FRTapomixisfluorescence sortingsite‐specific recombinationspecific promoter

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Area of Science:

  • Plant Biotechnology
  • Genetics
  • Agricultural Science

Background:

  • Apomictic hybrid rice development is hindered by challenges in inducing high rates of apomixis and distinguishing clonal from zygotic embryos.
  • Accurate selection of clonal seeds is critical for advancing apomictic rice cultivation.

Purpose of the Study:

  • To develop and validate a novel dual-fluorescence labelling gene switch system for distinguishing clonal seeds in apomictic hybrid rice.
  • To assess the efficacy of the pollen-specific gene switch (PSGS) system in improving clonal seed purity.

Main Methods:

  • Development of a pollen-specific gene switch (PSGS) system using Cre/LoxP + FRT recombination under a pollen-specific promoter.
  • Co-transformation of PSGS vectors with apomixis vectors into hybrid rice varieties (Yongyou 2640 and Yongyou 4949) via Agrobacterium-mediated transformation.
  • Analysis of seed fluorescence and ploidy in T1 and T2 generations to evaluate PSGS accuracy.

Main Results:

  • The PSGS system successfully identified mutants capable of distinguishing between clonal and zygotic seeds.
  • Specific lines (L47-4 and L151-1) produced non-fluorescent (diploid) seeds, indicating successful segregation.
  • The PSGS system demonstrated a sorting accuracy rate of 80.2% to 88.9% for clonal seed selection.

Conclusions:

  • The developed PSGS system is effective in differentiating clonal from zygotic seeds in hybrid rice.
  • This technology is essential for enhancing clonal seed purity and facilitating the advancement of apomixis in rice breeding.
  • PSGS offers a reliable tool for researchers and breeders working on apomictic rice development.