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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
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Non-destructive seed genotyping via microneedle-based DNA extraction.

Mingzhuo Li1, Aditi Dey Poonam1, Qirui Cui2

  • 1Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA.

Plant Biotechnology Journal
|March 20, 2025
PubMed
Summary
This summary is machine-generated.

A new microneedle (MN) patch rapidly isolates DNA from crop seeds without harming germination. This non-destructive method enables quick, in-field genotyping for improved crop breeding and food security.

Keywords:
DNA extractionbreedingmicroneedleseed genotypingsequencing

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Genetics

Background:

  • Crop breeding is crucial for food security, but traditional genotyping methods are slow and costly.
  • Limitations in current genotypic evaluations hinder efficient crop improvement programs.

Purpose of the Study:

  • To develop a rapid, non-destructive, and in-field DNA extraction platform for crop seeds.
  • To enable instant marker analysis and facilitate crop breeding advancements.

Main Methods:

  • A handheld microneedle (MN)-based platform using polyvinyl alcohol (PVA) MN patches was developed for DNA extraction from soybean seeds.
  • A 3D-printed device was designed for multiplexed DNA extraction in a microplate format.

Main Results:

  • Successful DNA extraction from softened soybean seeds using PVA MN patches.
  • High seed viability was maintained post-sampling (82% vs. 79% germination).
  • Extracted DNA quality supported PCR, LAMP, and whole-genome sequencing, enabling identification of genetic differences between varieties.

Conclusions:

  • The MN patch technology offers a rapid, non-destructive method for in-field crop seed DNA extraction and genotyping.
  • This platform has the potential for large-scale application to accelerate crop breeding and enhance food security.