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Non-destructive seed genotyping via microneedle-based DNA extraction.
Mingzhuo Li1, Aditi Dey Poonam1, Qirui Cui2
1Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA.
Plant Biotechnology Journal
|March 20, 2025
Summary
A new microneedle (MN) patch rapidly isolates DNA from crop seeds without harming germination. This non-destructive method enables quick, in-field genotyping for improved crop breeding and food security.
Area of Science:
- Agricultural Science
- Biotechnology
- Genetics
Background:
- Crop breeding is crucial for food security, but traditional genotyping methods are slow and costly.
- Limitations in current genotypic evaluations hinder efficient crop improvement programs.
Purpose of the Study:
- To develop a rapid, non-destructive, and in-field DNA extraction platform for crop seeds.
- To enable instant marker analysis and facilitate crop breeding advancements.
Main Methods:
- A handheld microneedle (MN)-based platform using polyvinyl alcohol (PVA) MN patches was developed for DNA extraction from soybean seeds.
- A 3D-printed device was designed for multiplexed DNA extraction in a microplate format.
Main Results:
- Successful DNA extraction from softened soybean seeds using PVA MN patches.
- High seed viability was maintained post-sampling (82% vs. 79% germination).
- Extracted DNA quality supported PCR, LAMP, and whole-genome sequencing, enabling identification of genetic differences between varieties.
Conclusions:
- The MN patch technology offers a rapid, non-destructive method for in-field crop seed DNA extraction and genotyping.
- This platform has the potential for large-scale application to accelerate crop breeding and enhance food security.
