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Jidangga-7 ameliorates non-small cell lung cancer by regulating gut microbiota function
Changcheng Yue1, Chula San1, Shichao Deng1
1Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China.
Objective:
This study aims to assess the effects of Jidangga-7 on enhancing gut microbiota function in non-small cell lung cancer.
Materials And Methods:
Eighteen mice were screened and randomly divided into three groups: a control group, a model group with induced non-small cell lung cancer, and a treatment group receiving Jidangga-7. A549 tumor cells were implanted in the mice, and tumor formation was monitored. Upon successful tumor induction, the treatment group received Jidangga-7 via oral gavage, while the other groups received an equivalent volume of saline. After the final dose, intestinal tissues were collected from each group, and microbial amplicon 16S analysis and non-extensive targeted metabolomics were employed to characterize intestinal fiber and associated metabolites.
Results:
By quantifying the contribution of individual species to the variations between the groups, the Sipmer results highlighted the top 10 species and their abundance that contribute to the differences between the two groups. Specifically, Jidangga-7 demonstrated a regulatory effect on various taxa such as Gammaproteobacteria, Bacilli, and Desulfovovoviridae. At the family level, administration of Jidangga-7 exhibited a regulatory effect on families including Desulfovibrionaceae, Lachnospiraceae, and Eggerthellaceae, compared to the model group. In untargeted metabolomics analyses, principal component analysis effectively differentiated the groups from one another. Subsequently, metabolites with a variable importance in projection score > 1 were screened. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed 20 metabolite pathways, encompassing metabolism of cofactors and vitamins, bacterial metabolism, antimicrobial pathways, and xenobiotics biodegradation and metabolism.
Conclusion:
Jidangga-7 exerted a positive influence on the intestinal microbial environment in mice with non-small cell carcinoma, ameliorating the dysbiosis induced by non- small cell lung cancer. This intervention inhibited the growth of pathogenic bacteria while fostering the growth of beneficial strains.
Insights
Jidangga-7 improves gut microbiota in non-small cell lung cancer (NSCLC) mice by regulating beneficial and pathogenic bacteria. This study shows Jidangga-7
Area of Science:
- Microbiology
- Oncology
- Pharmacology
Background:
- Non-small cell lung cancer (NSCLC) is associated with gut dysbiosis.
- Altering gut microbiota may offer therapeutic strategies for NSCLC.
Purpose of the Study:
- To evaluate the efficacy of Jidangga-7 in modulating gut microbiota function in a mouse model of NSCLC.
Main Methods:
- NSCLC was induced in mice, which were then treated with Jidangga-7 or saline.
- Gut microbial composition was analyzed using 16S rRNA sequencing.
- Metabolomic profiling identified key metabolic pathways affected by Jidangga-7.
Main Results:
- Jidangga-7 administration altered the abundance of specific bacterial taxa, including Gammaproteobacteria and Bacilli.
- The treatment regulated key microbial families such as Desulfovibrionaceae and Lachnospiraceae.
- Metabolomic analysis revealed significant changes in pathways related to cofactor metabolism, bacterial metabolism, and xenobiotic degradation.
Conclusions:
- Jidangga-7 positively influences the intestinal microbial environment in NSCLC mice.
- The compound ameliorates NSCLC-induced gut dysbiosis by inhibiting pathogens and promoting beneficial bacteria.
