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Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation

Fatma Doğan Güzel1,2, Hamed Ghorbanpoor3

  • 1Department of Mechanical Engineering, Faculty of Engineering and Natural Science, Ankara Yıldırım Beyazıt University, Ankara, Turkey. fdogan@aybu.edu.tr.

Journal of Fluorescence
|March 20, 2025
PubMed
Summary

This study reveals critical flow regimes for Deoxyribonucleic acid (DNA) in microfluidic channels. Understanding these DNA flow dynamics is key for lab-on-chip applications.

Keywords:
DNA behaviour in FlowFlow-dominated regimeFluorescence Correlation Spectroscopy (FCS)MicrofluidicsRheological properties of DNA

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Area of Science:

  • Biophysics
  • Chemical Engineering
  • Polymer Science

Background:

  • Investigating biopolymer behavior, like Deoxyribonucleic acid (DNA), in flow is crucial for engineering, polymer, and life sciences.
  • Real-time analysis of DNA rheological properties and flow characteristics presents significant challenges.

Purpose of the Study:

  • To investigate the flow dynamics and rheological properties of DNA in microfluidic environments.
  • To characterize the diffusion- and flow-dominated regimes for DNA molecules in microfluidic channels.

Main Methods:

  • Utilized confocal detection combined with Fluorescence Correlation Spectroscopy (FCS) for real-time velocity measurements.
  • Conducted optical experiments to analyze molecular behavior under varying flow conditions.

Main Results:

  • Identified the flow-dominated regime for DNA, commencing at a flow rate of 0.3 µl/min.
  • Characterized the transitional flow regime for DNA, occurring between 0.02-0.3 µl/min.

Conclusions:

  • The study provides valuable insights into the flow dynamics of DNA in microfluidic systems.
  • Findings are significant for the development and optimization of lab-on-chip devices and DNA analysis techniques.