Related Experiment Video
Updated: May 21, 2025
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017
The effects of intragraft CD38+ B cell on chronic active antibody mediated rejection in kidney transplantation
Yu Hui1,2, Linkun Hu2, Liangliang Wang2
1Department of Urology, The Fourth Affiliated Hospital of Soochow University, Suzhou, 215000, China.
Purpose:
This study aimed to determine the relation between CD38+ B-cell infiltration and outcome of transplant recipients diagnosed with chronic active antibody mediated rejection (caABMR).
Methods:
A total of 67 patients transplanted from April 2003 and July 2021 with biopsy-proved caABMR were included. The density of CD3+ , CD20+ and CD38+ cells were quantified by immunohistochemical staining and baseline information, estimated glomerular filtration rate (eGFR) and 24 h urine quantitative protein at diagnose were collected. Receiver operating characteristic (ROC) curves were used to calculate the cut-off values in the prediction of graft loss and prognosis factors were further analyzed by Cox proportional-hazards analysis. Kaplan-Meier death-censored graft survivals of subgroups divided by CD38+ B-cell infiltration or urine quantitative protein were compared.
Results:
The median density of CD3+ , CD20+ and CD38+ cells was 278, 167 and 89 (per mm2). CD38+ B cell infiltration in biopsies was associated with graft survival and the cut-off value was ≥ 93/mm2 with 92.90% sensitivity, 66.00% specificity, the cut-off values of 24-h urine quantitative protein and eGFR were ≥ 1.85 g and ≤ 29.50 ml/min*1.73m2 correspondingly, with 85.70%, 85.60% sensitivity, 64.20%, 74.90% specificity. Cox proportional-hazards analysis showed that the increase of CD38+ B cell infiltration and urine protein were independent risk factors and eGFR was a protective factor for caABMR with the hazard ratios of 1.96 (95%CI 1.03-3.72, P = 0.040), 1.24 (95%CI 1.01-1.52, P = 0.042) and 0.94 (95%CI 0.89-0.99, P = 0.025) correspondingly. Kaplan-Meier survival analysis demonstrated that patients with CD38+ B-cell infiltration ≥ 93/mm2 or 24-h urine protein ≥ 1.85 g had significantly reduced graft survival (P < 0.01).
Conclusion:
A threshold of CD38+ B cell infiltration ≥ 93/mm2 was associated with poor graft survival and CD38 might be a potential therapeutic target of caABMR.
