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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jul 14, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Reagentless aptamer based on the ultrasensitive and fast response electrochemical capacitive biosensor for EGFR

Enkhzaya Ganbold1, Nam Young Kim2, Yu Mi Kim3

  • 1RFIC Bio Centre, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea; Department of Electronics Engineering, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea; NDAC Centre, Kwangwoon University, 20 Kwangwoon-ro, Nowon-Gu, Seoul 01897, Republic of Korea.

Biosensors & Bioelectronics
|March 20, 2025
PubMed
Summary

A new electrochemical biosensor detects epidermal growth factor receptor (EGFR) mutations, crucial for early non-small cell lung cancer (NSCLC) diagnosis. This rapid, label-free method offers a promising tool for point-of-care cancer diagnostics.

Keywords:
Biomedical applicationEGFR detection in non-small cell lung cancerRapid detectionRegenerable aptamerReusable interdigitated capacitor electrode

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Diagnostics

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading global cause of cancer-related mortality.
  • Epidermal growth factor receptor (EGFR) mutations are prevalent in NSCLC, particularly in Asian populations, highlighting their importance in diagnosis.
  • Early detection of NSCLC is critical for improving patient outcomes.

Purpose of the Study:

  • To develop a novel electrochemical capacitive biosensor for the early detection of NSCLC.
  • To specifically identify epidermal growth factor receptor (EGFR) as a biomarker for NSCLC.
  • To create a rapid, label-free, and highly selective diagnostic tool for point-of-care applications.

Main Methods:

  • Designed a reagentless EGFR aptamer using systematic evolution of ligands by exponential enrichment (SELEX).
  • Immobilized the aptamer on a chromium/gold (Cr/Au) interdigitated capacitor electrode (IDCE) functionalized with 3-mercaptopropionic acid (MPA).
  • Utilized 6-mercapto-1-hexanol (MCH) for blocking and employed capacitance signals for real-time detection.

Main Results:

  • Achieved rapid, real-time detection of EGFR within 3 seconds.
  • Demonstrated a low detection limit of 0.005 ng/mL for EGFR peptide with a wide dynamic range (10^-11 to 10^-7 ng/mL).
  • The biosensor showed regenerability and reusability up to five times.

Conclusions:

  • The developed IDCE capacitive biosensor provides a highly selective and sensitive method for early-stage EGFR detection in NSCLC.
  • This label-free, rapid, and regenerable biosensor is a promising candidate for point-of-care diagnostic applications in biomedicine.
  • The technology has the potential to significantly advance cancer diagnostics and improve patient management.