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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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Mechanistic insights into CDCA gene family-mediated glioblastoma progression: implications for diagnosis, prognosis,

¹School of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518116, China. liu1208882672@163.com.

|March 21, 2025

Summary

Cell Division Cycle-Associated (CDCA) genes are overexpressed in glioblastoma (GBM), acting as potential diagnostic biomarkers and therapeutic targets. Their elevated expression correlates with poor prognosis and tumor progression in GBM patients.

Keywords:

CDCA genes Diagnosis Glioblastoma Immune cells Treatment

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Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Glioblastoma (GBM) is an aggressive brain tumor with limited treatment options.
Understanding GBM molecular drivers is crucial for developing new therapies.
Cell Division Cycle-Associated (CDCA) genes are understudied in GBM and may influence proliferation and treatment resistance.

Purpose of the Study:

To investigate the pathogenic, diagnostic, and therapeutic roles of CDCA genes in GBM.
To explore CDCA genes as potential biomarkers and therapeutic targets for GBM.

Main Methods:

RT-qPCR was used to analyze CDCA gene expression in GBM cell lines.
Bioinformatic analyses (TCGA, mutation, CNV, methylation) assessed prognostic and genetic roles.
Functional assays (gene knockdown, proliferation, migration) evaluated CDCA7 and CDCA8 in U87MG cells.

Main Results:

CDCA genes were significantly overexpressed in GBM cell lines and tumors compared to normal brain tissue.
CDCA gene expression demonstrated excellent diagnostic potential for GBM.
Higher CDCA gene expression correlated with poor prognosis and was linked to cancer-related pathways and immune modulation.
Knockdown of CDCA7 and CDCA8 reduced GBM cell proliferation, colony formation, and migration.

Conclusions:

CDCA genes are promising diagnostic biomarkers for GBM.
CDCA genes represent potential therapeutic targets for GBM treatment.