A Novel ToBRFV cDNA Full-Length Infectious Clone Provides Insights on Virus-Host Range and Inoculation Strategies

Area of Science:

• Plant Virology
• Molecular Biology
• Agricultural Science

Background:

• Tomato brown rugose fruit virus (ToBRFV) poses a significant threat to global tomato production.
• Effective research tools are crucial for understanding and managing ToBRFV.

Purpose of the Study:

• To construct a full-length infectious clone of ToBRFV.
• To characterize the infectivity and host range of the constructed clone.
• To provide a valuable tool for ToBRFV research and disease management.

Main Methods:

• Gibson Assembly was used to create the full-length ToBRFV clone (pJL89-ToBRFV).
• Agro-inoculation and mechanical inoculation were employed to infect various host plants.
• Host range studies were performed to identify susceptible species.

Main Results:

• The infectious clone successfully induced systemic infections in Nicotiana benthamiana, N. tabacum 'Samsun nn', and Solanum lycopersicum 'Linda'.
• Infectious virions, identical to wild-type, were produced in infected plants.
• Physalis floridana and Claytonia perfoliata were identified as new hosts for ToBRFV.

Conclusions:

• The pJL89-ToBRFV infectious clone is a functional tool for ToBRFV research.
• This clone facilitates studies on virus-host interactions and resistance screening.
• It aids in the development of ToBRFV control agents and attenuated strains.