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Channel Rhodopsins

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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

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Published on: June 10, 2020

Directed Evolution of a Genetically Encoded Indicator for Chloride.

Weicheng Peng^1,2, Jasmine N Tutol¹, Shelby M Phelps¹

¹Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, Texas 75080, United States.

ACS Synthetic Biology

|March 21, 2025

Summary

Researchers engineered a new fluorescent protein indicator for chloride using directed evolution. This advancement expands the utility of green fluorescent protein (GFP) variants for biological research.

Area of Science:

Biochemistry
Molecular Biology
Protein Engineering

Background:

Keywords:

GFP anions chloride sensing directed evolution fluorescence microscopy fluorescent indicator

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The green fluorescent protein (GFP) family is a versatile tool in biological research and protein engineering.

Chloride indicators based on GFP have been developed over the past 25 years.

Previous efforts utilized site-directed and combinatorial mutagenesis, but directed evolution remains largely untapped for chloride sensing.