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Updated: Sep 29, 2026
Murine Model of Wound Healing
Published on: May 28, 2013
Enhanced Wound Healing with Rhodiola rosea Phytosomal Gel: Extraction, Formulation, and In vivo Evaluation
Anandarajagopal Kalusalingam1, Abdullah Khan2, Mahibub Mahamadsa Kanakal2
1School of Pharmacy, KPJ Healthcare University, Nilai, 71800, Negeri Sembilan, Malaysia.
Background:
This study explores the formulation and effectiveness of a Rhodiola rosea phytosomal gel for enhancing wound healing.
Objective:
The ethanolic extract of Rhodiola rosea roots, rich in bioactive compounds like alkaloids and flavonoids, was optimized into a phytosomal complex to improve absorption and dermal retention.
Methods:
Characterization through GC-MS revealed compounds, such as 2-Heptadecenal and Bicyclo[ 4.1.0]Heptane and 7-Pentyl. FTIR confirmed the successful encapsulation within the phospholipid bilayer, while SEM showed smooth, spherical particles.
Results:
The Box-Behnken design optimized formulation parameters, achieving high yield (92.64%), small particle size (355 nm), and high entrapment efficiency (93.98%). In vitro release studies displayed a consistent release profile, aligning with Zero-order and Hixson-Crowell models. In vivo evaluation on Wistar rats showed that the phytosomal gel significantly enhanced wound healing, achieving 98.16% wound reduction by day 14, compared to 95.17% for R. rosea extract and 97.13% for standard treatment. Histopathological analysis demonstrated complete tissue regeneration and well-organized collagen fibers in the phytosomal gel group.
Conclusion:
This research highlights the potential of Rhodiola rosea phytosomal gel as an effective wound healing therapy, with future studies suggested for extended stability tests and human skin permeation studies.
