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Area of Science:

  • Biotechnology
  • Laboratory Safety
  • Aerosol Science

Background:

  • Cell sorters can generate hazardous aerosols during instrument failures, posing risks to operators.
  • Current aerosol containment relies on evacuation systems and biosafety cabinets, but evacuation systems require regular verification.
  • Operator safety is paramount when handling infectious or hazardous samples in cell sorting.

Purpose of the Study:

  • To describe an updated protocol for verifying the containment and evacuation of aerosols from cell sorters.
  • To ensure aerosol evacuation systems are operational and provide adequate protection before processing hazardous samples.
  • To validate the effectiveness of engineering controls in preventing operator exposure to aerosols.

Main Methods:

  • Simulating instrument failure by disrupting the cell sorter stream and generating aerosols.
  • Using 1-µm fluorescent beads to create detectable aerosol particles.
  • Collecting air samples outside the sort collection area with impactor-style aerosol samplers.
  • Examining collected samples for the presence of fluorescent beads to detect aerosol leakage.

Main Results:

  • Absence of beads in air samples indicates adequate aerosol containment and evacuation.
  • Presence of beads signifies a potential failure in the aerosol evacuation system or other controls.
  • The protocol provides a user-driven verification method for aerosol evacuation systems.

Conclusions:

  • Regular verification of aerosol evacuation systems is crucial for operator safety.
  • The described protocol offers a reliable method to detect aerosol containment failures.
  • Ensuring proper function of aerosol evacuation systems is essential for safe laboratory practices when using cell sorters.