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Updated: May 21, 2025
A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
Published on: January 27, 2019
Low levels of CD39+ Tregs may predict poor outcome in children with sepsis
Yanchun Ji1, Chenhao Wang1, Bo Wang2
1Department of Critical Care Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China; National Clinical Research Center for Child Health and Disorders, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China; Chongqing Key Laboratory of Pediatric Metabolism and Inflammatory Diseases, Chongqing 400014, China.
Objectives:
Sepsis is a life-threatening condition caused by a dysregulated immune response. This study aimed to explore immune dysregulation in pediatric sepsis and identify prognostic biomarkers for mortality prediction.
Methods:
Ninety-nine children with sepsis were followed for 28 days. Flow cytometry was used to analyze T cells, T-helper cells, B cells, natural killer (NK) cells, dendritic cells (DCs), and their subpopulations. Correlations between CD39+ regulatory T cells (Tregs), plasmacytoid DCs (pDCs), and transitional B cells with Phoenix Sepsis Score (PSS) were assessed. Receiver operating characteristic and Kaplan-Meier analyses were used to evaluate the prognostic value of CD39+ Tregs and pDCs. Cytokine levels were measured using a cytometric bead array.
Results:
CD39+ Tregs and transitional B cells were significantly lower in the nonsurvival and remote groups, while pDCs showed the opposite trend. CD39+ Tregs were negatively correlated with PSS, and pDCs were positively correlated. CD39+ Tregs below 12.45 % and pDCs above 0.96 % were both strong predictors of increased mortality risk. CD39+ Tregs maintained stable predictive performance within 72 h.
Conclusions:
CD39+ Tregs and pDCs may serve as potential prognostic biomarkers in pediatric sepsis, with specific thresholds predicting increased mortality risk.
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