Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translation01:31

Translation

14.3K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
14.3K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.0K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.0K
Ribosome Profiling02:24

Ribosome Profiling

3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K
Leaky Scanning02:28

Leaky Scanning

5.0K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.0K
Ribosomes01:27

Ribosomes

66.9K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
66.9K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic discovery of motif-based interactions of the auxiliary domains of USP family deubiquitinases.

Nature communications·2026
Same author

The TcVps34-TcVps15 complex regulates parasite metacyclogenesis and host cell infection in <i>Trypanosoma cruzi</i>.

Frontiers in cellular and infection microbiology·2026
Same author

Ancestral Wolbachia lineages are likely donors of ribotoxin genes in Aedes aegypti.

Journal of evolutionary biology·2026
Same author

Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids.

PloS one·2026
Same author

A tryptophan-phenylalanine binding motif for the histone methyltransferases MLL4 and MLL3.

The Journal of biological chemistry·2026
Same author

Structure-Based Discovery of Non-Covalent Triazole-Based Cruzipain Inhibitors with Improved Bioactivity Translation.

Journal of chemical information and modeling·2025

Related Experiment Video

Updated: May 21, 2025

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

7.9K

Trypanosoma brucei L19 is essential for ribosomal function.

Juan Manuel Zárate1, Leandro Simonetti2, María Jimena Manzur1

  • 1Área de Biología Molecular, Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas IMIBIO-SL, San Luis, Argentina.

Biochemical and Biophysical Research Communications
|March 21, 2025
PubMed
Summary

This study identifies unique features in Trypanosoma brucei ribosomal protein L19 (TbL19), crucial for parasite survival. Its distinct structure offers a potential new target for antiparasitic drug development.

Keywords:
18S rRNAExpanded segmentsRibosomal protein L19RibosomeT. brucei

More Related Videos

Examination of the Telomere G-overhang Structure in Trypanosoma brucei
15:25

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Published on: January 26, 2011

14.2K
Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor
08:50

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor

Published on: January 19, 2024

439

Related Experiment Videos

Last Updated: May 21, 2025

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

7.9K
Examination of the Telomere G-overhang Structure in Trypanosoma brucei
15:25

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Published on: January 26, 2011

14.2K
Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor
08:50

Author Spotlight: Advancements in Glycosomal pH Monitoring in Trypanosoma brucei Using pHluorin2 Biosensor

Published on: January 19, 2024

439

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Parasitology

Background:

  • Ribosomes evolve distinct structural variations, enabling targeted drug development.
  • Trypanosomatids, early diverging eukaryotes, possess unique protein synthesis machinery components.

Purpose of the Study:

  • To investigate the evolutionary divergence of ribosomal proteins in Trypanosomatids.
  • To characterize the structure and function of Trypanosoma brucei ribosomal protein L19 (TbL19).
  • To assess TbL19 as a potential drug target against trypanosomiasis.

Main Methods:

  • RNA interference (RNAi) for gene silencing.
  • Functional complementation assays in yeast.
  • Comparative structural analysis of ribosomal proteins.

Main Results:

  • TbL19 exhibits unique C-terminal domains involved in ribosome subunit interactions.
  • TbL19 is essential for Trypanosoma brucei survival.
  • TbL19 cannot functionally replace its yeast counterpart, confirming evolutionary divergence.

Conclusions:

  • TbL19's distinct structure is specific to Trypanosoma brucei and Trypanosoma cruzi.
  • This unique feature presents a promising target for novel antiparasitic drug discovery.