NIR-activating glycyrrhizic acid/carbon nanozyme injectable polysaccharides-based hydrogels for promoting...

The slow healing or non-healing of skin wounds caused by polymicrobial infections has become a serious problem in clinical wound treatment. Herein, we have developed a near-infrared (NIR) activating glycyrrhizic acid/carbon nanozyme injectable polysaccharides-based hydrogel (the CPCA hydrogel) for the synergistic treatment of polymicrobial infected wound. The CPCA hydrogel could undergo phase transition at a specific temperature and facilitate administration at the wound site. Additionally, under near-infrared light irradiation, the CPCA hydrogel could generate heat and promote the release of glycyrrhizic acid (GA) for achieving photothermal-drug synergistic treatment of multiple bacteria. Furthermore, the carbon nanozyme (CN) within the injectable polysaccharides-based hydrogel could mimic the activity of superoxide dismutase (SOD) and catalase (CAT) for enabling the removal of reactive oxygen species, effectively alleviating inflammation and promoting wound healing caused by polymicrobial infections. The results of in vitro antibacterial experiments demonstrated the excellent antibacterial effect of CPCA hydrogel on methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Furthermore, in vivo experiments confirmed that the hydrogels significantly reduced inflammatory responses and accelerated angiogenesis in polymicrobial infected wounds. Collectively, the CPCA hydrogel exhibited excellent antibacterial and anti-inflammatory properties, offering a novel strategy for developing new treatments for polymicrobial infections.