Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil-to-lymphocyte ratio is associated with diabetic peripheral neuropathy in type 2 diabetes patients.

Diabetes research and clinical practice·2017
Same author

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers.

Scientific reports·2017
Same author

Tetramethylpyrazine blocks TFAM degradation and up-regulates mitochondrial DNA copy number by interacting with TFAM.

Bioscience reports·2017
Same author

Mitochondrial LON protease-dependent degradation of cytochrome c oxidase subunits under hypoxia and myocardial ischemia.

Biochimica et biophysica acta. Bioenergetics·2017
Same author

The efficacy and safety of epinephrine for postoperative bleeding in total joint arthroplasty: A PRISMA-compliant meta-analysis.

Medicine·2017
Same author

The correlation between histological gastritis staging- 'OLGA/OLGIM' and serum pepsinogen test in assessment of gastric atrophy/intestinal metaplasia in China.

Scandinavian journal of gastroenterology·2017

Related Experiment Video

Updated: Jun 12, 2025

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.8K

Visualize neuronal membrane cholesterol with split-fluorescent protein tagged YDQA sensor.

Yi Xu1, Saixuan Li1, Yiran Xu1

  • 1State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, PR China.

Journal of Lipid Research
|March 21, 2025
PubMed
Summary

Researchers developed a novel sensor to visualize neuronal cholesterol in vivo. This tool revealed distinct cholesterol changes in Alzheimer's disease models, offering new insights into neurological disorders.

Keywords:
Alzheimer’s diseasecholesterolplasma membranesensor

More Related Videos

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
12:58

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Published on: November 20, 2012

13.1K
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
08:31

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Published on: July 29, 2021

1.5K

Related Experiment Videos

Last Updated: Jun 12, 2025

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.8K
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
12:58

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Published on: November 20, 2012

13.1K
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
08:31

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Published on: July 29, 2021

1.5K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Cholesterol is vital for neuronal plasma membrane (PM) function and brain health.
  • Disrupted cholesterol homeostasis is linked to neurological disorders like Alzheimer's disease (AD).
  • In vivo visualization of neuronal cholesterol remains a significant challenge in understanding AD pathology.

Purpose of the Study:

  • To develop and validate a novel sensor for detecting neuronal PM cholesterol in vivo.
  • To investigate cholesterol distribution changes in mouse models of AD.

Main Methods:

  • Generation of a split-fluorescent protein-based sensor (sfPMcho) using a cholesterol-dependent cytolysin derivative (YDQA).
  • Validation of the sfPMcho sensor in cell and C. elegans models.
  • Application of the sfPMcho sensor in 5X FAD and APOE KO mouse models of AD.

Main Results:

  • The sfPMcho sensor effectively detects neuronal PM cholesterol in vivo.
  • Significant alterations in cholesterol distribution were observed in AD mouse models.
  • Specifically, PM cholesterol was sparse and aggregated in neuron bodies but accumulated in nerve fibers.

Conclusions:

  • The sfPMcho sensor provides a valuable new tool for in vivo detection of neuronal PM cholesterol.
  • This study uncovers specific cholesterol abnormalities at the cellular level in AD-related pathology.
  • Further development of such sensors is expected to advance AD research.