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Cellular inactivation by heat and shear.

F Dunn

Radiation and Environmental Biophysics

|January 1, 1985

Summary

This study shows that combining heat and shear stress inactivates Chinese hamster V79 cells. These combined stresses mimic ultrasound effects, suggesting shear stress is a key factor in ultrasound-induced cell death.

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Area of Science:

Cellular biology
Biophysics
Biomaterials

Background:

Ultrasound is used for various medical applications, but its effects on cells, particularly inactivation, are not fully understood.
Previous studies suggest a combination of thermal and mechanical stresses contribute to ultrasound-induced cell death.
Investigating the specific role of shear stress in cell inactivation is crucial for optimizing ultrasound therapies.

Purpose of the Study:

To investigate the in vitro inactivation of Chinese hamster V79 cells using combined thermal elevation and Couette shear flow.
To mimic shear stress conditions produced by ultrasound (3 MHz, 3 W/cm2) used in previous cellular studies.
To compare the effects of combined shear and thermal stress with those of ultrasound and thermal stress on cell survival.

Main Methods:

Chinese hamster V79 cells were exposed to a temperature of 43°C.
Cells were subjected to Couette shear flow designed to replicate ultrasound-induced shear stresses.
Cell survival curves were generated to analyze the combined effects of heat and shear stress.

Main Results:

The combination of thermal stress (43°C) and Couette shear flow resulted in cell inactivation.
The observed survival curves demonstrated a summating effect between shear and thermal stresses.
The inactivation patterns closely resembled those previously reported for ultrasound and thermal stress combined.

Conclusions:

Shear stress, in conjunction with thermal stress, significantly contributes to the inactivation of Chinese hamster V79 cells.
The findings suggest that shear stress is a critical component of ultrasound-mediated cellular inactivation.
This research provides insights into the mechanisms of ultrasound bioeffects and aids in the development of safer and more effective ultrasound applications.

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