Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 21, 2025

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

18.4K

Spatiotemporal transcriptome atlas of developing mouse lung.

Xiaogao Meng1, Wenjia Li2, Jian Xu3

  • 1Life Science and Medicine, University of Science and Technology of China, Hefei 230026, China; Center for Cell Lineage Technology and Bioengineering, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, China-New Zealand Belt and Road Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.

Science Bulletin
|March 21, 2025
PubMed

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An interpretable, clinically-aligned AI paradigm for VTE risk prediction: an approach using LLMs and compound attention.

Computer methods in biomechanics and biomedical engineering·2026
Same author

Organoid modeling of lung branching morphogenesis and epithelial lineage specification.

Developmental cell·2026
Same author

IAPEz retrotransposons regulate the first lineage segregation in mouse pre-implantation development.

Nucleic acids research·2026
Same author

MeDiCNet: Integrating Multi-scale Dynamic Convolution and Enhanced Position-Aware Transformer for DNA Methylation Site Prediction.

Interdisciplinary sciences, computational life sciences·2026
Same author

Developmental chronology of mouse embryo from 2-cell stage through birth.

Nature cell biology·2026
Same author

Author Correction: Modelling late gastrulation in stem cell-derived monkey embryo models.

Nature·2026
Summary
This summary is machine-generated.

This study maps mouse lung development using spatial transcriptomics, revealing key molecular domains and gene networks critical for airway and alveolar formation. The findings offer insights into respiratory development and regeneration.

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Mammalian lung development requires precise spatial and temporal coordination of diverse cell types.
  • A comprehensive spatiotemporal transcriptome atlas of lung development has been lacking.

Purpose of the Study:

  • To create a detailed molecular atlas of mouse lung development from E12.5 to P0.
  • To identify critical spatial domains, lineage trajectories, and regulatory networks involved in lung organogenesis.
  • To characterize alveolar niche heterogeneity and identify key genes and signaling pathways in alveologenesis.

Main Methods:

  • Application of high-throughput spatial transcriptomics.
  • Integration of published single-cell RNA-sequencing data.
  • Analysis of lineage trajectories and transcription factor (TF) regulatory networks.
Keywords:
Alveolar nicheAlveologenesisBranching morphogenesisLung developmentProximal–distal patterningRegulatory networkSpatial molecular lineageSpatial transcriptomics

More Related Videos

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging
04:16

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging

Published on: April 19, 2024

879
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

727

Related Experiment Videos

Last Updated: May 21, 2025

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

18.4K
Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging
04:16

Author Spotlight: Advancing Lung Transplant Immunology Through Intravital Imaging

Published on: April 19, 2024

879
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

727

Main Results:

  • Identification of 10 critical spatial domains for lung organization.
  • Characterization of proximal-distal patterning in developing pulmonary airways.
  • Discovery of differentially expressed genes (e.g., Angpt2, Epha3) and enriched signaling pathways (ANGPT, VEGF, EPHA) in the maturing alveolar niche.

Conclusions:

  • The study provides a comprehensive spatiotemporal molecular atlas of mouse lung development.
  • This atlas details molecular domains, cell communication, and regulatory networks.
  • The findings are crucial for understanding human lung development and advancing respiratory regeneration medicine.