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Updated: May 21, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The high-quality chromosome-level genome assembly of Dracocephalum rupestre Hance
Qian Zhao1,2, Zixu Fan1, Hui Yu3,4
1Inner Mongolia Key Laboratory of Disease-Related Biomarkers, The Second Affiliated Hospital, Baotou Medical College, Baotou, 014030, China.
Abstract:
Dracocephalum rupestre Hance is China's traditional herbal medicine in the family Labiatae with numerous health benefits, including anti-inflammatory, antiviral and anti-tumor activities. However, the genus Dracocephalum has no reference genome currently, which restricts the research on the breeding, cultivation and exploration of medicinal properties in D. rupestre. Thus, we present the high-quality chromosome-level genome assembly of D. rupestre using a combination of Pacbio HiFi sequencing and Hi-C scaffolding technologies. The final genome was 435.45 Mb with a contig N50 of 49.83 Mb and a scaffold N50 of 59.06 Mb. The assembled sequences were anchored to 7 chromosomes with an integration efficiency of 96.96%. Furthermore, we predicted 25,865 protein-coding genes, 98.23% of which were functionally annotated. These results offer valuable resources for understanding the genetic basis of the unique phenotypes of D. rupestre and will facilitate further study of the functional genomics of this species.
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