The chromosome-level genome assembly of Broad-Leaf Fern (Dipteris shenzhenensis)

Dipteris is a relic plant genus and an important indicator of global climate warming and plant geography during the Mesozoic era. However, the lack of genomic resources has hindered the study of paleoclimate, systematic evolution, and medicinal value of this genus. Here, we sequenced and assembled the first chromosome-level genome of Dipteris shenzhenensis. The assembled genome was 1.9 Gb with a contig N50 length of 4.75 Mb, GC content of 42.28% and BUSCO value of 98.3%, and 98.37% of the assembled sequences were anchored onto 33 pseudochromosomes. 71.97% of the genome were predicted to be repetitive sequences, and 45 telomeres were identified, including 15 paired telomeres. A total of 26,471 protein coding genes were predicted, of which 24,485 (92.5%) genes were functionally annotated. The first high-quality genome of Dipteris will provide important genome resources for understanding the systematic evolution, paleoclimate and medicinal value of ferns.