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Engineering Yarrowia lipolytica for Enhanced Gastrodin Production via High-Throughput Screening and
Mengchen Hu1, Yijin He1, Yifan Ma1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China.
Biotechnology and Bioengineering
|March 22, 2025
Summary
High-throughput screening and transcriptomics successfully enhanced microbial gastrodin production. Optimized strains achieved a 13.1 g/L gastrodin titer, paving the way for industrial applications.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Microbial Production
Background:
- Gastrodin, a key compound from Gastrodia elata, is vital in medicine and nutraceuticals.
- Current microbial production methods require enhancement for industrial viability.
Purpose of the Study:
- To improve microbial gastrodin production using high-throughput screening (HTS) and transcriptomics.
- To identify and overexpress key genes for increased gastrodin yield.
Main Methods:
- Atmospheric pressure and room temperature plasma (ARTP)-mediated mutagenesis for strain development.
- Established an HTS method using a transcription factor-based biosensor and HP-SPE-MS.
- Transcriptomics analysis to identify target genes for overexpression.
Main Results:
- Isolated mutant strain MT8 with a 55.6% increase in gastrodin production (9.8 g/L).
- Overexpression of gene YALI2E01737g further increased gastrodin to 10.1 g/L.
- Fermentation optimization resulted in a final gastrodin titer of 13.1 g/L.
Conclusions:
- HTS and transcriptomics are effective strategies for enhancing gastrodin production.
- The developed microbial strain and process show potential for industrial-scale gastrodin synthesis.
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