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Related Experiment Video

Updated: May 21, 2025

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix

Published on: July 10, 2016

Three-dimensional rosettes in epithelial formation.

Lila Neahring¹, Jennifer A Zallen¹

¹HHMI and Developmental Biology Program, Sloan Kettering Institute, New York, NY, United States of America.

Cells & Development

|March 22, 2025

Summary

This summary is machine-generated.

Multicellular rosettes are key to building epithelial tissues. These structures coordinate cell behavior, enabling the formation of diverse tissue architectures essential for development and disease understanding.

Keywords:

Apical-basal polarity Cell adhesion Epithelial morphogenesis Lumenogenesis Mesenchymal-epithelial transition Myosin contractility Rosette

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Area of Science:

Developmental Biology
Cell Biology
Tissue Engineering

Background:

Epithelial tissues perform vital structural, signaling, and barrier functions.
Understanding how individual cells form collective epithelial structures is a key developmental biology question.
Multicellular rosettes are emerging as critical intermediates in epithelial tissue formation.

Purpose of the Study:

To review the mechanisms regulating rosette assembly and dynamics.
To discuss the role of rosettes as versatile intermediates in epithelial morphogenesis.
To highlight open questions in rosette biology and their implications for development and disease.

Main Methods:

Literature review of recent studies on multicellular rosettes.
Analysis of cellular and molecular mechanisms governing rosette formation and behavior.
Discussion of biophysical principles underlying rosette dynamics.

Main Results:

Radially polarized rosettes provide a protected environment for cells to develop polarity and adhesion.
Rosettes dynamically expand, move, and coalesce to generate various epithelial structures.
Rosettes are fundamental to forming epithelial sheets, tubes, cavities, and branched networks.

Conclusions:

Multicellular rosettes are crucial for coordinating cell interactions and building complex epithelial tissues.
Rosette dynamics are essential for diverse morphogenetic processes during embryonic development.
Further research into rosette mechanisms can illuminate embryonic development and human diseases.