Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nephrons

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...

Renal Corpuscle

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Translation, Cross-Cultural Adaptation and Validation of the Serbian Version of the Clinical Frailty Scale in Patients Undergoing Major Uro-Oncological Surgery.

Healthcare (Basel, Switzerland)·2026

Same author

Incidence and Perioperative Risk Factors for Postoperative Delirium After Major Urological Surgery.

Diagnostics (Basel, Switzerland)·2025

Same author

Impact of Epicatechin Supplementation on Plasma Proteome Profiles in Obese Men and Women-An Exploratory Approach to Sexual Dimorphism.

Proteomics. Clinical applications·2025

Same author

Shenfu injection promotes self-renewal of intestinal stem cells in sepsis-induced intestinal injury via inducing ATF4 expression.

Frontiers in pharmacology·2025

Same author

Does Green Tea Ameliorate Obesity in Mice Kept at Thermoneutrality by Modulating Skeletal Muscle Metabolism?

Cell biochemistry and function·2025

Same author

The choice of diet is determinative for the manifestation of UCP1-dependent diet-induced thermogenesis.

American journal of physiology. Endocrinology and metabolism·2025

Search research articles

Related Experiment Video

Updated: May 21, 2025

Author Spotlight: Developing a Bedside Protocol for Kidney and Genitourinary Ultrasonography

Author Spotlight: Developing a Bedside Protocol for Kidney and Genitourinary Ultrasonography

Published on: June 21, 2024

No UCP1 in the kidney.

Celso Pereira Batista Sousa-Filho¹, Natasa Petrovic¹

¹Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.

Molecular Metabolism

|March 22, 2025

Summary

This summary is machine-generated.

Uncoupling protein 1 (UCP1) is not found in the kidney. Rigorous testing with appropriate controls invalidated previous reports suggesting kidney UCP1 expression, including findings from UCP1 reporter mice.

Keywords:

Antibody Brown adipose tissue Immunohistochemistry Kidney UCP1

More Related Videos

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Published on: April 25, 2025

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

Related Experiment Videos

Last Updated: May 21, 2025

Author Spotlight: Developing a Bedside Protocol for Kidney and Genitourinary Ultrasonography

Author Spotlight: Developing a Bedside Protocol for Kidney and Genitourinary Ultrasonography

Published on: June 21, 2024

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Unilateral Ureteral Obstruction Model for Investigating Kidney Interstitial Fibrosis

Published on: April 25, 2025

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Published on: March 4, 2022

Area of Science:

Physiology
Cell Biology
Metabolism

Background:

Recent studies suggested Uncoupling Protein 1 (UCP1) presence in the kidney.
This challenged the established understanding of UCP1 being confined to brown and beige adipocytes.
Previous findings were based on immunohistochemistry and reporter mouse studies.

Purpose of the Study:

To confirm or refute the presence of UCP1 in kidney tissue.
To physiologically characterize UCP1 expression in the kidney.

Main Methods:

Immunohistochemistry was performed on kidney sections using four different UCP1 antibodies.
Quantitative PCR (qPCR) was used to assess UCP1 mRNA levels.
Experiments included UCP1-ablated mice as negative controls.

Main Results:

Initial antibody testing showed positive signals in kidney tissue, consistent with prior reports.
However, these signals persisted in UCP1-ablated mice, indicating non-specific antibody binding.
Highly specific antibodies confirmed UCP1 presence only in perirenal adipose tissue, not the kidney. UCP1 mRNA was undetectable.

Conclusions:

There is no evidence for UCP1 presence in the kidney when proper controls are used.
Previous findings of kidney UCP1 expression require reconfirmation.
Results from UCP1 reporter mice regarding kidney expression need further validation.