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Enhanced expression of miR-128 in peripheral blood mononuclear cells: a potent candidate diagnostic biomarker for
Hossein Akhgari1, Alireza Ahmadi1, Abedeh Rezaei1
1Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Objectives:
Millions of people worldwide, especially young adults, are affected by multiple sclerosis (MS), a demyelinating and autoimmune condition of the central nervous system (CNS). Many studies have focused on the diagnostic value of miRNAs circulating in body fluids of MS patients. Thus, this study aimed to assess the expression of miR-128 and its potential as a biomarker for MS in the Iranian population.
Methods:
This study recruited 85 MS patients and 30 healthy matched controls. Following the collection of blood samples, serum was isolated. RNA was then extracted, cDNA was synthesized, and miR-128 expression was measured by real-time PCR.
Results:
There was significant overexpression of miR-128 in MS patients in comparison with healthy controls (p-value <0.0001). In addition, there was a rather notable association between the subtypes of MS samples and the expression levels of miR-128. ROC analysis showed an AUC of 0.9111 (95%CI 0.85-0.97, p-value <0.0001) with sensitivity and specificity of 80.00% and 93.33%, respectively.
Conclusion:
The expression of miR-128 was significantly higher in PBMCs of MS patients in comparison with healthy controls. Additionally, miR-128 could be an excellent biomarker for MS patient diagnosis.
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