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Single-cell sequencing combined with urinary multi-omics analysis reveals that the non-invasive biomarker PRDX5 regulates bladder cancer progression through ferroptosis signaling

  • 0Department of Urology, The Second Hospital of Lanzhou University, Lanzhou, 730000, China.
Bmc Cancer +

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March 24, 2025

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March 24, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Peroxidase 5 (PRDX5) is upregulated in bladder cancer (BC), promoting tumor growth by altering reactive oxygen species (ROS) and ferroptosis. Targeting PRDX5 may offer new BC treatment strategies.

Area Of Science

  • Oncology
  • Molecular Biology
  • Biochemistry

Background

  • Bladder cancer (BC) remains a significant health concern with complex underlying molecular mechanisms.
  • Understanding the role of specific proteins in BC progression is crucial for developing targeted therapies.

Purpose Of The Study

  • To investigate the expression profile and biological functions of peroxidase 5 (PRDX5) in bladder cancer.
  • To determine PRDX5's role in BC progression via modulation of reactive oxygen species (ROS) and ferroptosis.

Main Methods

  • Utilized The Cancer Genome Atlas (TCGA) data and urine proteomics for PRDX5 expression analysis.
  • Employed single-cell RNA sequencing (scRNA-seq) to assess PRDX5 distribution in the tumor microenvironment.
  • Conducted in vitro and in vivo experiments to evaluate PRDX5's impact on BC cell behavior and ferroptosis pathways.

Main Results

  • PRDX5 was significantly upregulated in BC tissues and enriched in bladder epithelial cells, correlating with disease progression.
  • Overexpression of PRDX5 enhanced BC cell proliferation, migration, and invasion, while its knockout inhibited tumor growth and activated ferroptosis.
  • PRDX5 was confirmed to modulate ROS levels and influence ferroptosis signaling in both in vitro and in vivo models.

Conclusions

  • Elevated PRDX5 expression is a key driver of bladder cancer progression.
  • PRDX5 regulates tumor growth by controlling ROS levels and ferroptosis pathways.
  • PRDX5 represents a potential therapeutic target for bladder cancer treatment.

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