Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
Transgenic Plants02:50

Transgenic Plants

7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
What is Genetic Engineering?00:49

What is Genetic Engineering?

73.0K
Overview
73.0K
Transgenic Organisms00:53

Transgenic Organisms

30.8K
Overview
30.8K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

18.7K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.7K
CRISPR01:59

CRISPR

48.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of genetically modified soybean FG72 for renewal authorisation under Regulation (EC) No 1829/2003 (dossier GMFF-2025-33580).

EFSA journal. European Food Safety Authority·2026
Same author

Analysis of transcriptomic alterations induced by 33 different per- and polyfluoroalkyl substances (PFAS) in differentiated HepaRG cells.

Archives of toxicology·2026
Same author

Methods2AOP: A Collaboration to Strengthen the Integration of Test Methods into the Adverse Outcome Pathway Framework.

F1000Research·2026
Same author

Rye Straw Lignin as a Promising Source of Tricin: Varietal Differences and Its Release Using Deep Eutectic Solvents.

ACS sustainable chemistry & engineering·2026
Same author

Assessment of additional information related to genetically modified oilseed rape MS11 (application EFSA-GMO-BE-2016-138).

EFSA journal. European Food Safety Authority·2026
Same author

Development of a QSAR model for predicting PPARα activation by PFAS based on human in vitro data of a comprehensive panel of legacy and novel PFAS.

Archives of toxicology·2026

Related Experiment Video

Updated: May 20, 2025

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

20.9K

Risk assessment considerations for RNAi-based genetically modified plants.

, Francisco Barro, Albert Braeuning

    EFSA Journal. European Food Safety Authority
    |March 24, 2025
    PubMed
    Summary
    This summary is machine-generated.

    This guidance outlines risk assessment requirements for genetically modified plants (GMPs) using RNA interference (RNAi) technology. It details necessary analyses for molecular characterization, food and feed safety, and exposure assessments for RNAi-based GMP applications.

    Keywords:
    RNA interferencegenetically modified organismsmolecular characterisationnon‐coding RNAoff‐targetrisk assessment

    More Related Videos

    Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
    09:57

    Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

    Published on: December 28, 2016

    10.6K
    Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
    10:14

    Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

    Published on: May 4, 2018

    13.8K

    Related Experiment Videos

    Last Updated: May 20, 2025

    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
    09:44

    RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

    Published on: December 21, 2015

    20.9K
    Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
    09:57

    Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

    Published on: December 28, 2016

    10.6K
    Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
    10:14

    Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

    Published on: May 4, 2018

    13.8K

    Area of Science:

    • Genetically Modified Organisms (GMOs)
    • Plant Biotechnology
    • Molecular Biology
    • Risk Assessment

    Background:

    • Regulation (EU) No 503/2013 and EFSA guidance (2011) define risk assessment (RA) for genetically modified plants (GMPs).
    • Genetically modified plants utilizing RNA interference (RNAi) for transcript silencing require specific additional analyses.
    • Existing strategies for assessing RNAi off-targets in plants need updating based on current knowledge.

    Purpose of the Study:

    • To provide updated guidance on risk assessment requirements for RNAi-based GMP applications submitted to EFSA.
    • To detail necessary analyses for molecular characterization, food/feed safety, and dietary exposure assessment.
    • To replace the previous GMO panel strategy for assessing RNAi off-targets.

    Main Methods:

    • Focus on bioinformatic analysis for molecular characterization.
    • Confirmation of the specific RNAi-induced trait.
    • Assessment of food and feed safety.
    • Evaluation of dietary exposure.

    Main Results:

    • Establishes comprehensive requirements for RNAi-based GMP applications.
    • Integrates current scientific understanding of RNAi mechanisms in plants.
    • Provides a unified approach to molecular, safety, and exposure assessments.

    Conclusions:

    • The guidance ensures robust risk assessment for RNAi-based GMPs.
    • It addresses specific challenges related to RNAi technology in plant genetic modification.
    • This updated strategy supports the safe development and deployment of RNAi-based GMPs.