Related Experiment Video

Updated: May 20, 2025

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Reduced high-density lipoprotein antioxidant function in patients with coronary artery disease and acute coronary

Benjamin Sasko1,2, Linda Scharow3, Rhea Mueller2

  • 1Ruhr-University of Bochum, Medical Department II, Marien Hospital Herne, Bochum, Germany.

JCI Insight
|March 24, 2025
PubMed

Insights

Reduced HDL antioxidant function is linked to coronary artery disease (CAD). The nHDLox marker strongly predicts acute coronary syndrome (ACS), highlighting its role in cardiovascular pathogenesis.

Area of Science:

  • Cardiovascular Science
  • Biochemistry
  • Clinical Medicine

Background:

  • High-density lipoprotein (HDL) function, not just concentration, is crucial in coronary artery disease (CAD) pathogenesis.
  • Reduced HDL antioxidant function is implicated in cardiovascular disease.
  • The study investigated the association between HDL antioxidant function and stable CAD or acute coronary syndrome (ACS).

Purpose of the Study:

  • To determine if reduced HDL antioxidant function is associated with stable CAD.
  • To assess if reduced HDL antioxidant function is associated with acute coronary syndrome (ACS).
  • To evaluate nHDLox as a predictor for ACS and CAD.

Main Methods:

  • Assessed HDL antioxidant function using a cell-free biochemical assay measuring HDL-lipid peroxide content (HDLox).
  • Normalized HDLox by HDL-C levels and a healthy pooled serum control to derive nHDLox.
  • Analyzed nHDLox in two cohorts: elective coronary angiography patients (n=1225) and ACS patients (n=196).

Main Results:

  • Participants with CAD (n=723) showed 12% higher mean nHDLox levels than those without CAD (n=502; P < 0.001).
  • Patients with ACS exhibited the highest nHDLox values compared to the elective cohort (median 1.35, IQR 0.97-1.85; P < 0.001).
  • Multivariate analysis confirmed nHDLox as a strong independent predictor of ACS (P < 0.001) but not of CAD (P > 0.05).

Conclusions:

  • HDL antioxidant function is diminished in patients with CAD.
  • The nHDLox marker is strongly associated with acute coronary syndrome (ACS).
  • nHDLox may serve as a valuable biomarker for identifying patients at risk of ACS.
Abstract

Keywords:
AtherosclerosisCardiologyCholesterolInflammationLipoproteinsMetabolism

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