Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Regulation01:08

Allosteric Regulation

57.3K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
57.3K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.3K
2.3K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.6K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.7K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

13.8K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A pH-regulated camouflage strategy for access control in molecular systems.

Nanoscale horizons·2026
Same author

DNA-based cooperative games: an interactive collective decision-making architecture.

Organic & biomolecular chemistry·2026
Same author

Designing DNA Triplexes with High Affinity and Specific Recognition Based on Multiple Biophysical Mechanisms.

Journal of chemical theory and computation·2025
Same author

Programmable DNA Hairpin Locker: Dual-Layer Encrypted Carrier Communication.

ACS nano·2025
Same author

pH-Controlled DNA Switching Circuits with Multi-State Responsiveness for Logic Computation and Control.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

DNA domino circuits based on a hairpin exonuclease assistance signal transmission architecture for temporal logic operations.

Chemical communications (Cambridge, England)·2024

Related Experiment Video

Updated: May 20, 2025

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.2K

An EHairpin-driven double-stem-loop programmable allosteric strategy for molecular security access control.

Yufeng Wang1, Xiaokang Zhang2, Peijun Shi2

  • 1Key Laboratory of Advanced Design and Intelligent Computing, Ministry of Education, School of Software Engineering, Dalian University, Dalian 116622, China. zhangq@dlu.edu.cn.

Nanoscale
|March 24, 2025
PubMed
Summary

This study introduces a novel DNA nanotechnology strategy for molecular security access control. The EHairpin-driven system offers a programmable, multi-level security solution for DNA devices, enhancing information security.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K

Related Experiment Videos

Last Updated: May 20, 2025

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.2K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.1K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Information Security

Background:

  • DNA nanotechnology offers non-computational complexity for information security, countering threats to modern cryptography.
  • Existing molecular security methods face limitations due to stringent experimental demands and complex DNA sequence design.

Purpose of the Study:

  • To propose an EHairpin-driven double-stem-loop programmable allosteric strategy for molecular security access control.
  • To develop a method for regulating molecular conformational changes in response to specific DNA input signals.
  • To establish a molecular-switch-response circuit for handling multiple input signals.

Main Methods:

  • Designed an EHairpin structure to control double-stem-loop conformational changes programmatically.
  • Converted molecular conformational changes into signal-response triggering events.
  • Constructed a molecular-switch-response circuit for temporal signal response and multiple inputs.

Main Results:

  • Successfully implemented an EHairpin-driven molecular security access control system.
  • Achieved a three-level security assurance mechanism: administrator authentication, authorization, and user authentication.
  • Demonstrated programmable allosteric regulation through specific DNA input signals.

Conclusions:

  • The proposed strategy provides a robust method for security access control in molecular devices.
  • This advancement promotes next-generation information security and offers new paradigms for nanomachines.
  • The technology holds significant potential for applications in biosensing and disease diagnosis.