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Synthesis and characterization of protein nanohybrid systems for the brain delivery of Riluzole
Ujjwal Gupta1, Amit Kumar1, Md Imtiyaz Alam1
1Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER) Raebareli, Lucknow, India.
Aims:
Synthesis and Characterization of Protein NanoHybrid Systems for the Brain Delivery of Riluzole.
Methods/Materials:
Fullerene is converted into carboxylated fullerene (CF) and then, prepared RZU-loaded BSA nanoparticles conjugated with CF.
Results:
The particle size and zeta potential of RZU-PNH were found to be 210 ± 1.15 nm and -18.5 ± 0.615 mV respectively, and entrapment efficiency and loading efficiency of RZU-PNH were found to be 98.8 ± 0.53% and 11.6 ± 0.43%, respectively. The XRD of the RZU-PNH shows the amorphism behavior and CD revealed that secondary structure of the protein mainly consists of α-helix andβ-sheet. The MTT assay showed 88.60% and 90.84% cell viability in both SH-SY5Yand N2a cell lines at a concentration of 20 μg/ml and also, no significant nasal ciliotoxicity was observed after incubation with RZU-PNH.
Conclusions:
Obtained results indicated RZU-PNH formulation to treat amyotrophic lateral sclerosis.
