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Vessel-On-A-Chip Coupled Proteomics Reveal Pressure-Overload-Induced Vascular Remodeling
Yanjun Liu1, Jianxujie Zheng1, Lingyan Zhong1
1Department of Chemistry, Zhongshan Hospital, Fudan University, Shanghai, 200000, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 24, 2025
Summary
A novel vessel-on-a-chip model accurately simulates hypertension, revealing how vessel stretch affects vascular smooth muscle cells (VSMCs). This technology aids in understanding cardiovascular disease and developing new hypertension treatments.
Area of Science:
- Biomedical Engineering
- Cardiovascular Research
- Cell Biology
Background:
- Hypertension is a major cause of cardiovascular disease and mortality.
- It involves increased vessel stretch and vascular smooth muscle cell (VSMC) alterations.
Purpose of the Study:
- To develop and validate a vessel-on-a-chip model for simulating hypertension.
- To investigate VSMC responses to physiological and pathological stretch.
- To compare chip findings with a mouse model of hypertension.
Main Methods:
- Development of a vessel-on-a-chip system.
- Application of proteomics analysis to VSMCs.
- Comparison of chip data with a mouse model of hypertension.
Main Results:
- Physiological stretch upregulated contractile markers in VSMCs.
- The chip replicated pathological stretch responses, including ERK signaling, calcium transport, integrin signaling, ER stress, TLR activation, oxidative stress, and sphingolipid synthesis.
- Chip findings correlated with in vivo events in the mouse model.
Conclusions:
- The vessel-on-a-chip system effectively mimics hypertension-related biological events.
- This model shows promise for studying vascular diseases.
- It can facilitate the development of novel hypertension therapeutics.
