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Assessment of Nucleic Acid Quality in Unstained Cytology Specimens for Cancer Genomic Testing
Hiroya Niimi1, Takafumi Onishi2,3, Shoma Nomura4
1Department of Health Sciences, Graduate School of Health Sciences, Kyoto Tachibana University, Kyoto, Japan, h901123007@st.tachibana-u.ac.jp.
Introduction:
The use of cytological specimens in cancer genome medicine has garnered considerable attention, but the long-term quality of nucleic acids from unstained specimens remains unclear. This study aimed to evaluate the quality of nucleic acids extracted from unstained specimens fixed with 95% ethanol or spray fixation over varying durations.
Methods:
Two lung cancer cell lines were prepared using the auto-smear method and fixed with 95% ethanol, and spray-fixed specimens were stored for 30 min, 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months, and 6 months. DNA was extracted using a DNA extraction kit, and quality was assessed using agarose gel electrophoresis and PCR.
Results:
Nucleic acids extracted from unstained specimens showed no fragmentation after 6 months of fixation and were amplifiable by PCR, regardless of the fixation method.
Conclusion:
Nucleic acids extracted from unstained specimens preserved high quality over 6 months, suggesting that such specimens are suitable for genetic testing. This finding has significant implications for the long-term storage and clinical application of cytological specimens in cancer genome medicine.
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