Related Experiment Video
Updated: Aug 3, 2026

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
Published on: February 27, 2014
Abstract:
A new plastic embedding medium for light microscopy is described. The monomer mixture consists of equal proportions by volume of acrylonitrile, dimethyl acrylamide and methyl methacrylate, and may be polymerized by exposure to ultraviolet light in the presence of benzoin methyl ether as catalyst. Dithiothreitol may also be added to the monomer mix to limit the degree of polymerization. The resulting polymer is soluble in dimethyl formamide.
More Related Videos
08:52High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials
Published on: July 7, 2017
07:41Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Published on: October 17, 2018
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...