From initial formation to developmental refinement: GABAergic inputs shape neuronal subnetworks in the primary...

Neuronal subnetworks, also known as ensembles, are functional units formed by interconnected neurons for information processing and encoding in the adult brain. Our study investigates the establishment of neuronal subnetworks in the mouse primary somatosensory (S1) cortex from postnatal days (P)11 to P21 using in vivo two-photon calcium imaging. We found that at P11, neuronal activity was highly synchronized but became sparser by P21. Clustering analyses revealed that while the number of subnetworks remained constant, their activity patterns became more distinct, with increased coherence, independent of cortical layer or sex. Furthermore, the coherence of neuronal activity within individual subnetworks significantly increased when synchrony frequencies were reduced by augmenting gamma-aminobutyric acid (GABA)ergic activity at P15/16, a period when the neuronal subnetworks were still maturing. Together, these findings indicate the early formation of subnetworks and underscore the pivotal roles of GABAergic inputs in modulating S1 neuronal subnetworks.