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Genome sequences of four A1 subcluster Mycobacterium smegmatis bacteriophages

Spencer T Payne1, Jayden S Longhurst1, Elisa A Correa Lazaro1

  • 1Department of Microbiology, Brigham Young University, Provo, Utah, USA.

Microbiology Resource Announcements
|March 25, 2025
PubMed

Insights

Four bacteriophages, Payneful, Marchy, Hami1, and Sorpresa, were identified as A1 subcluster tailed phages infecting Mycobacterium smegmatis. Their genomes share characteristics with other A1 phages and contain six new open reading frames.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria.
  • Mycobacterium smegmatis is a commonly studied non-pathogenic mycobacterium.
  • The A1 subcluster comprises tailed bacteriophages within the Caudoviricetes class.

Purpose of the Study:

  • To characterize four novel bacteriophages: Payneful, Marchy, Hami1, and Sorpresa.
  • To determine their taxonomic classification within the A1 subcluster.
  • To analyze their genomic features and identify potential novel genes.

Main Methods:

  • Isolation and characterization of bacteriophages infecting Mycobacterium smegmatis mc²155.
  • Genome sequencing and analysis.
  • Comparative genomics with known A1 subcluster phages.

Main Results:

  • Payneful, Marchy, Hami1, and Sorpresa were confirmed as A1 subcluster tailed bacteriophages.
  • Their genome length and GC content align with established A1 subcluster phage characteristics.
  • Six previously undiscovered open reading frames (ORFs) were identified within their genomes.

Conclusions:

  • The identified phages represent new members of the A1 subcluster infecting Mycobacterium smegmatis.
  • Genomic analysis reveals conserved traits and novel genetic elements.
  • These findings contribute to the understanding of mycobacteriophage diversity and evolution.
Keywords:
Cluster A1

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