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Abstract:
Pilot-plant scale experiments using production scale fractionation equipment have been carried out to prepare human serum albumin according to a two-step simple method reported previously. Results have indicated that scale-up is feasible and the resultant albumin is of at least the same quality but with better yield when compared to that obtained by the conventional ethanol fractionation method. Filtration to replace centrifugation in one of the steps for liquid-solid separation was also attempted. It proved to be superior to centrifugation in terms of time and labor savings, but a small percentage of albumin was not recoverable. However, because of the simplicity and higher yield of this fractionation method the final albumin yield is still higher than that obtained by the conventional ethanol method even if the filtration step is introduced. Diafiltration and ultrafiltration are used for solvent removal and concentration, instead of lyophilization and reconstitution to prepare the final albumin solutions.