Related Experiment Video

Updated: May 20, 2025

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
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Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT

Published on: January 22, 2018

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Effect of PRKD3 on cell cycle in gastric cancer progression and downstream regulatory networks

Shuaiyang Wang1,2, Bei Xie3, Haohua Deng1,2

  • 1Department of Clinical Laboratory Center, Lanzhou University Second Hospital, Lanzhou, 730000, Gansu, China.

Medical Oncology (Northwood, London, England)
|March 25, 2025
PubMed

Insights

Protein kinase D3 (PRKD3) is overexpressed in gastric cancer (GC), driving tumor progression and adverse patient outcomes. Inhibiting PRKD3 reduces GC malignancy and offers a potential therapeutic target for this disease.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein kinase D3 (PRKD3) is implicated in tumor development, but its specific role in gastric cancer (GC) and cell cycle regulation remains unclear.
  • Understanding PRKD3's function is crucial for developing targeted GC therapies.

Purpose of the Study:

  • To investigate the role of PRKD3 in gastric cancer progression and its impact on the cell cycle.
  • To identify potential therapeutic strategies targeting PRKD3 in GC.

Main Methods:

  • Bioinformatic analysis of PRKD3 expression in GC tissues.
  • In vitro experiments using GC cell lines to assess the effects of PRKD3 knockdown on malignancy.
  • Proteomic and Western blot analyses to elucidate PRKD3's molecular mechanisms.

Main Results:

  • PRKD3 was significantly overexpressed in GC tissues, correlating with poor patient prognosis.
  • PRKD3 knockdown suppressed GC cell proliferation, migration, and invasion, inducing G2/M phase arrest.
  • Proteomic analysis revealed PRKD3 affects cell cycle proteins (CDK1, CyclinB1, CHK1, PLK1) and signaling pathways (FoxO, p53).

Conclusions:

  • PRKD3 overexpression is linked to aggressive GC behaviors and poor outcomes.
  • Targeting PRKD3 presents a promising therapeutic avenue for gastric cancer treatment.
Keywords:
Cell cycleGastric cancerPRKD3Tumor progression

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