miR-200b-3p affects the proliferation and differentiation of chicken preadipocytes by modulating SESN1 expression...

Excessive deposition of abdominal fat in chickens has a significant impact on the poultry industry, and there is increasing evidence that non-coding RNAs play a crucial role in fat development. In our previous RNA-seq study, miR-200b-3p was found to be differentially expressed during different developmental periods of fat in Gushi chickens. In this study, we report that miR-200b-3p can directly bind to the 3'UTR region of SESN1 to promote proliferation and inhibit differentiation of preadipocytes. Overexpression of SESN1 inhibits preadipocyte proliferation and promotes differentiation. In contrast, inhibition of SESN1 resulted in the opposite outcome. Interestingly, we also identified the circADGRF5/miR-200b-3p/SESN1 ceRNA network involved in the developmental process of preadipocytes. Furthermore, we validated a novel circRNA, circADGRF5, in this report and found that it regulates SESN1 expression through competitive binding with miR-200b-3p. In conclusion, these data suggest that miR-200b-3p directly targets SESN1 to regulate the proliferation and differentiation of preadipocytes, and circADGRF5 regulates SESN1 expression through competitive binding with miR-200b-3p.