Understanding the role of poly(3-hydroxybutyrate) depolymerases in waste management

Since conventional plastics are poorly degradable and harmful to living organisms, there is a need to look for alternative options such as poly(3-hydroxybutyrate) - PHB. This polymer is biodegradable via PHB depolymerases (PHBDs). Literature survey reveals that there are no comprehensive updates on PHBDs and in the current review, details regarding extracellular and intracellular forms of the enzyme are presented. Phylogenetically diverse PHBD-producing microorganisms are prevalent in a variety of natural and man-made habitats. These enzymes have been purified from different organisms and are seen to be active over a wide range of pH and temperatures. In general, extracellular PHBDs are made up of four constituents - signal peptide, catalytic domain [with a signature triad (serine, aspartate and histidine) and an oxyanion histidine], linker domain and substrate binding domain that enable degradation of PHB. Intracellular PHBDs have been mainly studied in bacterial genera such as Rhodobacter, Rhodospirillum, Ralstonia, Zoogloea, Pseudomonas, Sinorhizobium and Bacillus. Genes encoding them have been identified, characterized and cloned in other organisms. PHBD producers are envisioned to play a significant role in developing ecofriendly methods for removing PHB wastes thereby highlighting their practical relevance. Development of appropriate consortia including diverse PHBD producers and their introduction in waste disposal sites would enable treatment of PHB containing wastes. By using bioinformatics and synthetic biology-based approaches, further investigations on PHBDs from unexplored microorganisms can be undertaken and their role in the bioplastic waste management can be unraveled.