Dispersive solid phase extraction of imatinib from human plasma using biological metal-organic and covalent-organic...

In this study, a dispersive solid-phase extraction method was developed using a metal organic framework and covalent organic framework composite for the efficient extraction of imatinib from human plasma before its determination by a high-performance liquid chromatography-tandem mass spectrometry detector. The composite was synthesized using cobalt chloride and nicotinic acid through a hydrothermal method, while the covalent organic framework was prepared from benzidine and terephthalaldehyde and deposited onto the metal organic framework. The synthesized adsorbent was characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM), confirming its successful formation and structural properties. The method exhibited limits of detection of 1.1 ng mL^-1 in plasma and 0.67 ng mL^-1 in deionized water, quantification of 3.7 ng mL^-1 in plasma and 2.2 ng mL^-1 in deionized water, a linear range of 3.7-500 ng mL^-1 in plasma and 2.2-500 ng mL^-1 in deionized water, and an extraction recovery of 59 % demonstrating the method's suitability for reliable and precise quantification of imatinib in biological and aqueous matrices. The method's precision was investigated by evaluating the relative standard deviation and it was found to be ≤6.6 %.