Related Experiment Video
Updated: May 20, 2025
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
Published on: November 10, 2021
Platelet-rich plasma improves cyclophosphamide-induced interstitial cystitis in rat models through the toll-like
Yufan Wu1,2, Lei Chen3, Minzhe Xu4
1Department of Urology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Gusu District, Suzhou, 215000, JiangSu Province, China.
Objective:
To investigate the therapeutic effect of platelet-rich plasma (PRP) on a cyclophosphamide (CYP)-induced interstitial cystitis (IC) rat model.
Methods:
A CYP-induced IC rat model (75 mg/kg every 3 days, with a total of five injections) was used to evaluate the therapeutic effects of PRP. Here, PRP was administered via bladder irrigation (every 2 days, with a total of three irrigations), and bladder tissue was analysed for inflammation and histological changes. The toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signalling pathway was assessed using real-time quantitative polymerase chain reaction and ribonucleic acid sequencing. In addition, lipopolysaccharide (LPS)-induced SV-HUC-1 cells (10 μg/LPS and 2.5 mM adenosine triphosphate) were employed to investigate the inflammatory response and the effects of PRP on the TLR4/NF-κB signalling pathway.
Results:
The PRP treatment significantly improved the bladder tissue condition in the CYP-induced IC rat model, as evidenced by reduced inflammation and histological damage. The damage and shedding of the superficial epithelium of the bladder mucosa were notably decreased following PRP bladder instillation. Importantly, the expression of ZO-1, a key marker of epithelial integrity, was upregulated in PRP-treated rats, indicating enhanced bladder epithelial function. High-throughput analysis revealed that PRP alleviated bladder mucosal injury in the IC rat model through the TLR4/NF-κB signalling pathway. In LPS-induced SV-HUC-1 cells, PRP treatment also increased ZO-1 expression, decreased CDH1 expression and regulated the TLR4/NF-κB signalling pathway.
Conclusion:
Platelet-rich plasma treatment may improve the expression of ZO-1 and CDH1 in urinary epithelium in vitro by mediating the TLR4/NF-κB pathway, which is effective in the treatment of IC.
