Viability studies of bacterial strains exposed to nitrogen oxides and light in controlled atmospheric conditions

Airborne biological particles, such as pollen, fungi, bacteria, viruses, and plant or animal detritus, are known as bioaerosols. Understanding bioaerosols' behavior, especially their reaction to pollutants and atmospheric conditions, is crucial for addressing environmental and health issues related to air quality. Such complex investigations can benefit from experiments in controlled but realistic environments, such as the Atmospheric Simulation Chamber facility ChAMBRe (Chamber for Aerosol Modeling and Bio-aerosol Research). In this work, we report on the results of several experiments that were conducted at ChAMBRe using three strains of bacteria: E. coli, B. subtilis, and P. fluorescens. The goal of these experiments was to quantitively study how the culturability of these bacteria is affected by exposure to NO, NO₂, and light. The experimental approach was simple but carefully controlled: before being introduced into ChAMBRe, the bacteria samples were characterized using three different methods to determine the ratio of viable to total bacteria. The bacteria suspension was then aerosolized and introduced into ChAMBRe, where it was exposed to two different concentrations of NO and NO₂, in dark conditions and with simulated solar radiation. The culturability of the bacteria was assessed by collecting bacteria samples directly onto Petri dishes by an Andersen impactor at various time intervals after the end of injection. Finally, the formed bacteria colonies were counted after 24-48 h of incubation to measure their culturability and the temporal trend. The results show a reduction of culturability for all bacteria strains when exposed to NO₂ (from 50 to 70%) and to high concentrations of NO (i.e. around 30% at more than 1200 ppb) at concentration values higher than the typical urban ambient values. Even higher effects were observed exposing the bacteria strain to a proxy of solar light. The findings show how atmospheric simulation chambers help the comprehension of interactions between pollutants and bioaerosols in controlled atmospheric environments.