Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.3K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.3K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

733
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
733
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

473
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
473
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

617
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
617
Humoral Immune Responses01:36

Humoral Immune Responses

71.1K
Overview
71.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring Free Will Beliefs and Attitudes Towards Punishment in Norway, Sweden and the United Kingdom.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Spatial and Phenotypic Heterogeneity of ILC Subsets in Mouse Lung Under Type 2 Inflammatory Conditions.

European journal of immunology·2026
Same author

Novel Partial Loss-of-function STAT3-variant as Cause of Hyper-IgE-Syndrome in a Danish Family with Variable Expressivity.

Journal of clinical immunology·2026
Same author

The roles of the acetyltransferase domains of the chromatin regulators KAT6A and KAT6B in vivo.

Development (Cambridge, England)·2026
Same author

Perinatal mAChR-mediated activation of cortical subplate neurons elicits network activity driving basket cell differentiation.

Frontiers in cellular neuroscience·2026
Same author

The benchmarking and application of tag-degraders in vivo to validate therapeutic targets.

Nature communications·2026
Same journal

Research advances and application prospects of CAR-T therapy in the treatment of age-related diseases.

Frontiers in immunology·2026
Same journal

Machine learning-driven identification and immunohistochemical validation of an integrated immune-inflammatory phenotype for disease-free survival stratification in breast cancer.

Frontiers in immunology·2026
Same journal

Modified treatment protocol for pediatric systemic lupus erythematosus-associated hemophagocytic lymphohistiocytosis with central nervous system involvement: a case report.

Frontiers in immunology·2026
Same journal

Exploratory characterization of IgG1/IgG4 glycosylation and monocyte-derived dendritic cell responses in esophageal squamous cell carcinoma.

Frontiers in immunology·2026
Same journal

JAK-STAT pathway-associated skin diseases: a refined functional framework for inflammatory skin diseases.

Frontiers in immunology·2026
Same journal

Cross-talk among novel programmed cell death pathways: a decisive network in renal ischemia-reperfusion injury.

Frontiers in immunology·2026
See all related articles

Related Experiment Video

Updated: May 20, 2025

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

12.0K

Decoding plasma cell maturation dynamics with BCMA.

Sebastian R Schulz1, Shannon R Menzel1, Jens Wittner1

  • 1Division of Molecular Immunology, Internal Medicine 3, University Hospital Erlangen, Nikolaus-Fiebiger Center, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.

Frontiers in Immunology
|March 26, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new BCMA reporter mouse to track plasma cell development. This tool aids in understanding the factors influencing plasma cell maturation and longevity, crucial for antibody production.

Keywords:
BCMA (TNFRSF17)antibody-secreting cells (ASC)bone marrowplasma cellsspleensurvivalthymus

More Related Videos

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

14.9K
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

15.1K

Related Experiment Videos

Last Updated: May 20, 2025

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

12.0K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

14.9K
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

15.1K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Plasma cells are essential for antibody production after infection or vaccination.
  • Plasma cell heterogeneity in lifespan and metabolism is regulated by intrinsic and extrinsic factors.
  • B cell maturation antigen (BCMA) is known to support long-lived plasma cells.

Purpose of the Study:

  • To develop a novel reporter mouse model for tracking plasma cell terminal maturation.
  • To investigate the expression patterns of BCMA during plasma cell development.
  • To explore the role of the in vivo microenvironment in plasma cell maturation.

Main Methods:

  • Construction of a BCMA reporter mouse (BCMA:Tom) that labels antibody-secreting cells.
  • Utilizing flow cytometry and advanced imaging techniques for analysis.
  • Co-analysis with the Blimp1-GFP reporter to track plasma cell development.

Main Results:

  • BCMA:Tom expression was specific to antibody-secreting cells and varied by IgH isotype.
  • BCMA expression increased with plasma cell maturity.
  • The reporter system highlighted the importance of the in vivo microenvironment for complete plasma cell maturation.

Conclusions:

  • The BCMA:Tom reporter mouse is a valuable tool for studying plasma cell development and maturation.
  • This reporter facilitates deeper understanding of mechanisms regulating plasma cell heterogeneity and longevity.
  • Advanced tracking of plasma cells can be achieved using flow cytometry and imaging techniques.