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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Studying the Cytoskeleton

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: May 20, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

Single-cell parallel plate mechanics by side-view optical microscopy-assisted atomic force microscopy.

Mingyang Yang^1,2, Yanqi Yang^1,2, Lianqing Liu¹

¹State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences Shenyang 110016 China limi@sia.cn.

Nanoscale Advances

|March 26, 2025

Summary

This summary is machine-generated.

This study introduces a new atomic force microscopy (AFM) method for real-time, side-view mechanical analysis of single cells. This technique quantifies cell stiffness and adhesion forces, advancing mechanobiology research.

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Last Updated: May 20, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Published on: June 27, 2013

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Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells

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Published on: April 2, 2021

Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Cellular mechanical forces are vital in physiological and pathological processes.
Atomic force microscopy (AFM)-based force spectroscopy is a key tool for single-cell mechanical measurements.

Purpose of the Study:

To develop and validate a novel single-cell mechanical measurement method using side-view optical microscopy-assisted AFM.
To enable real-time visualization and quantification of uniaxial cell mechanics.

Main Methods:

A wedged probe was utilized with AFM and side-view optical microscopy for uniaxial force spectroscopy.
Whole-cell compression and adhesion assays were performed to measure Young's modulus and cell adhesion forces.

Main Results:

The method allowed real-time visualization of the AFM force spectroscopy process.
Young's modulus of cells was quantified, and the effects of experimental parameters and actin inhibitors were examined.
AFM-based uniaxial single-cell force spectroscopy (SCFS) successfully measured cell adhesion forces.

Conclusions:

The presented method offers a promising approach for comprehensive single-cell mechanical analysis.
This technique enhances the application of AFM force spectroscopy in mechanobiology.
It provides deeper insights into the role of mechanical cues in biological activities.